Title

TISSUE SPECIFIC MICRORNA MEAN EXPRESSION IN LIPOSARCOMA AND LIPOMA

PP-13

General

E-Poster Presenter

Faculty of medicine, Chulalongkorn University, Bangkok, Thailand

Author(s)

Namsiri R

Charoenlap C

Hongsaprabhas C

Vongpaisarnsin K

Thanakit V

Shuangshoti S

Background

Lipomatous tumors is common soft-tissue tumors. Diagnosis and differentiation between lipoma and liposarcoma subtype are mandatory for management and prognostic prediction. Multiple methods can distinguish between lipoma and liposarcoma including immunohistochemistry and messenger ribonucleic acid (mRNA), however they both have some disadvantages. Recent studies revealed that microRNA (miRNA) had an important role in the tumorigenesis, therefore it could be used as an aid for diagnosis, respond to treatment and development to targeted therapy.

Study Design & Methods

We performed a retrospective analytical study looking into the different expressions of mir-143, mir-144, mir-422a, mir-451 and mir-485-5p between lipoma and liposarcoma by using quantitative real-time polymerase chain reaction (PCR). All microRNAs were extracted from formalin-fixed paraffin-embedded (FFPE) sample of 65 liposarcomas including all subtypes compare with 13 lipomas. The expressions level of 5 microRNAs were analysed by TaqMan real-time PCR assays. Statistical analysis were determined by Mann-Whitney U-test and ROC curve.

Results

There was a 0.92–fold change decrease in expression of mir-143 (p=0.004), 0.91–fold change in mir-144 (p=0.004), 0.90–fold change in mir-422a (p=0.001), and 0.93–fold change in mir-451 (p=0.003) comparing between liposarcoma and lipoma. We also found that miRNAs decrease in expression of mir-143, mir-144, mir-422a, mir-451 were significantly lower in well-differentiated, dedifferentiated, myxoid-round cell, and unclassified liposarcomas compare to the lipoma. The change of expression in mir-485 was not significant in other subtype, except in unclassified liposarcoma with a 0.94-fold change (P=0.022) when compare with the lipoma.

Conclusions

The expression level of microRNA could serve as an available liposarcoma biomarker. We found that the under-expression level of mir-143 mir-144 mir-451 and mir-485-5p had diagnostic value for lipomatous tumors. In addition, microRNA is a powerful tool to discriminate liposarcoma subtypes. Hence, it can be used as an aid for diagnosis, respond to treatment and development to targeted therapy.

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