ANTIPROLIFERATIVE ASSESSMENT INDUCED BY LEAF EXTRACTS OF ANDROGRAPHIS PANICULATA, ZIZIPHUS SPINA-CHRISTI AND VERNONIA EXTENSA ON CANCER CELL LINES
Cancer is the most numerous health issues worldwide. Andrographis paniculata (AP), Ziziphus spina-christi (ZSC) and Vernonia extensa (VE) are well-known as medicinal herbs. Potentiate bioactivities of these plants are possible as the natural chemotherapeutic drugs.
Leaves of three plants were extracted with water, methanol, ethanol and dichloromethane at ratio 1:10 (w/v). The antiproliferative effects of three plants were determined by MTT assay on MCF-7 (human breast adenocarcinoma), HT-29 and SW-620 (human colorectal adenocarcinoma) and Vero (normal kidney cells) cells. The apoptotic was assessed using Annexin-V assay.
Results revealed that methanolic extract of AP had the highest antiproliferative effect towards MCF-7 cells with IC50 value of 10.92 ± 0.46 µg/ml. Ethanolic and dichloromethane extract of AP exhibited strong antiproliferation against SW-620 and HT-29 cells with IC50 values of 12.04 ± 1.15 and 12.91 ± 5.12 µg/ml, respectively. Other plants, ZSC extracted with dichloromethane exhibited high antiproliferation to MCF-7 cells with IC50¬ value of 12.99 ± 0.21 µg/ml. Hot water extract of AP exhibited antiproliferative effect to MCF-7 cells and induces high antiproliferation against Vero cells. Water extract of ZSC and VE induces antiproliferative effect to MCF-7 and SW-620 cells. The methanolic extract of AP showed the highest antiproliferative effect against MCF-7 cells. Therefore, apoptosis was determined by treated with IC50 of methanolic extract of AP. Methanolic extract of AP induces apoptosis as 33.94% within 24 hours. After decreasing of dose at 0.25, 0.5 of IC50 concentration and increasing incubation time at 48 hours, the apoptosis percentage were increased as dose-dependent manner
In conclusion, AP has the highest antiproliferative effect when extracted with methanol on MCF-7 cells. Moreover, methanol extract of AP inflicts apoptosis with a dose-dependent manner. Further studies about in vivo or molecular mechanisms induced by these plants chemical constituents are needed to be conducted.